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KMID : 0613820180280010090
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Journal of Life Science
2018 Volume.28 No. 1 p.90 ~ p.98
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Characteristics of Eggshell Powder as Carriers of Probiotics
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Lee Woo-Do
Niu Kai-Min
Lim Jeong-Min
Yi Kwon-Jung
Lee Bong-Joo
Kim Kang-Woong
Kim Kyoung-Duck
Hur Sang-Woo
Han Hyon-Sob
Kim Soo-Ki
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Abstract
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Eggshell (ES) is a by-product of table eggs with high content of calcium carbonate which can be used as a calcium source in feed. In this study, we have first illuminated the potential application of ES as a novel carrier for probiotics. The carriers used in the study include a SBM (Soybean meal), ESL (Eggshell powder with large particles), ESF (Eggshell powder with fine particles), and the complex carriers (SBM+ESL, SBM+ESF). The structure of carriers absorbed by L. plantarum was confirmed by SEM image. Among these carriers, the complex carrier SBM+ESF showed the highest viability of L. plantarum with pH 7~8 during four weeks storage at room temperature. The SBM+ESF was further tested as a carrier for various probiotic strains at 4℃ or 30℃. All the probiotic strains showed high viability at 4℃ storage. However, a significant reduction of Lactobacillus cells was observed at 30℃ storage. B. lichenifomis maintained high viability whereas B. subtilis, B. amyloliquefaciens, and S. cerevisiae showed the reduction of 2 log10 (CFU/g). These results suggest that if the ESF as a calcium source in feed was mixed with SBM, it can be used as an effective complex carrier for improving the viability of some probiotics including B. licheniformis.
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KEYWORD
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Carrier, eggshell powder, probiotics, SEM, viability
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